J. K. Wickiser Lab

Archive for the ‘Lab Business’ Category

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Gene expression network reconstruction by convex feature selection when incorporating genetic perturbations.

Monday, September 12, 2011

Generating or constructing the network is one thing, but it’s very difficult to ensure the model is predictive in terms of perturbations. This paper details efforts on how to do just that: Perturbations on Genetic Networks

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Free Speech and Public Service

Wednesday, September 7, 2011

Here’s a neat analysis detailing the balance of free speech and control as a gov’t employee.

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Friends’ labs

Monday, September 5, 2011

Here’s some new links to labs of my friends:

Wade Winkler’s new digs

Ann Miller’s new digs

John Rinn

Martin Schmeing (also a member of the middle name club)

Luke Rice

Mark Wilson

and others on the way

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M$ OneNote for iPad

Thursday, August 25, 2011

Ok, I’m a OneNote wonk. And I want an iPad. Here’s a new reason to dive in:

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Cool meeting in the near future.

Monday, August 22, 2011

NYAS meeting on synthetic biology.

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Naturally Obsessed – one of the best science documentaries I’ve seen

Friday, August 19, 2011

Naturally Obsessed What a sad, amazing, inspiring, and tragic look at the way science is actually conducted. This is how the pictures in the text books are made. We forget the human element behind it all. Also notice that this PI unwittingly – I can only hope – forces three grad students to race to a single endpoint.

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Meet the Scientists in the lab

Wednesday, August 17, 2011

My name is Matt Browne and I have been working in the JKW lab this summer. I am a rising junior at James I. O’Neill High School in Highland Falls and I live right here in wonderful West Point. Along with my laboratory compadres, I have been studying the effects that weapons contaminants have on the gene expression of several organisms, namely E. Coli and B. subtilis. My work in the lab includes bacterial culturing procedures, RNA and DNA extraction techniques, gel electrophoresis, reverse transcription to create cDNA from RNA, PCR and quantitative PCR, and world-champion level pipette skills. My favorite aspect of working in the lab is being able to come into an environment where I am always provided the opportunity to solve problems and really work out my critical thinking skills.

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Meet the scientists of JKW Lab

Tuesday, August 9, 2011

Hi my name is Hannah Lachance and I am a laboratory technician in the JKW lab. The main project I am working on involves studying how organisms such as E. coli and B. subtilis react to RDX exposure.

Techniques I use in these experiments includes growing cell cultures in various mediums, extracting RNA and DNA, running reverse transcriptions in order to obtain cDNA, using organism specific primers to run PCR and QPCR and performing gel electrophoresis. I highly enjoy working in the lab because it helps reinforce all that I have learned in school in a very practical and meaningful way. In addition, I get to work with amazing people and have fun while learning and accumulating valuable skills.

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R and Bioconductor Tutorials

Tuesday, February 1, 2011

http://www.cyclismo.org/tutorial/R/

http://www.biostat.harvard.edu/%7Ecarey/rr.pdf

http://mercury.bio.uaf.edu/mercury/R/R.html

http://www.r-project.org/

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Good undergrad cloning site

Tuesday, February 1, 2011

RIT Cloning Class Site

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